| 货号 | AF6677-SP |
| 别名 | CAD4; cadherin 4, type 1, preproprotein; cadherin 4, type 1, R-cadherin (retinal); cadherin-4; CDH4; FLJ22202; FLJ40547; MGC126700; MGC138355; RCAD; R-CAD; R-Cadherin; Retinal cadherin |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Cadherin-4/R-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Cadherin-4/R-Cadherin and recombinant mouse (rm) Cadherin-2 is observed and less than 1% cross-reactivity with rmE‑Cadherin is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1002 (Human); 12561 (Mouse); 311710 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-4/R-Cadherin His21-Ala731 Accession # P39038 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta -cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Cadherin‑4/R‑Cadherin by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cadherin‑4/R‑Cadherin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Cadherin‑4/R‑Cadherin in Mouse Brain. Cadherin‑4/R‑Cadherin was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
