| 货号 | AF6667-SP |
| 别名 | Beta-galactoside-binding lectin L-14-II; GAL2; Gal-2; galectin 2; galectin-2; HL14gal-2; Lactose-binding lectin 2; lectin, galactoside-binding, soluble, 2; LGALS2; MGC75071; S-Lac lectin 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Galectin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human Galectin-2 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3957 (Human); 107753 (Mouse); 171134 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Galectin-2 Ser2-Glu130 (Gly36Val) Accession # Q9CQW5 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Galectin-2 (Gal-2; also LEG2, HL14 and L-14-II) is a 14-15 kDa member of the S-type (1-CRD type) lectin family of proteins. It is a potentially secreted, noncovalent homodimer that is expressed by stomach mucosal columnar epithelium, small intestine Goblet cells and keratinocytes. Galectin-2 is reported to bind to CD29, and initiate CD8+ T cell apoptosis. Structurally, it serves to bind nonsialylated poly-NAcetyllactosamine motifs. Mouse Galectin-2 is 130 amino acids (aa) in length. There is one galectin domain (aa 4-130) that contains one dimerization interface (aa 123-128). Full-length mouse Galectin-2 shares 86% and 66% aa identity with rat and human Galectin-2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Galectin‑2 by Western Blot. Western blot shows lysates of mouse stomach tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Galectin‑2 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Galectin‑2 in Mouse Intestine. Galectin‑2 was detected in perfusion fixed frozen sections of mouse intestine using Mouse Galectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6667) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hemotoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
