| 货号 | AF6574-SP |
| 别名 | lipogenic protein 1; Lpgp; LPGP1; S14; S14MGC21659; Spot 14 protein; SPOT14; SPOT14SPOT14 homolog; thyroid hormone responsive (SPOT14 homolog, rat); thyroid hormone responsive SPOT14 (rat) homolog; thyroid hormone responsive SPOT14; thyroid hormone responsive; thyroid hormone-inducible hepatic protein | 全称 | Thyroid Hormone Responsive [SPOT14 Homolog] Protein |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects mouse THRSP in Western blots. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7069 (Human); 21835 (Mouse); 25357 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse THRSP Gly47-Leu150 Accession # Q62264 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | THRSP (Thyroid hormone inducible hepatic protein; also S14/SPOT 14) is an 18-20 kDa acidic nuclear protein that belongs to the SPOT14 family of molecules. It is expressed by lactating mammary epithelium, adipocytes and hepatocytes, and appears to promote the synthesis of long-chain fatty acids. Its synthesis is induced by thyroid hormone, insulin and carbohydrates. Mouse THRSP is 150 amino acids (aa) in length. The residues between aa 10-66 contain a Leu-zipper domain that mediates noncovalent homodimerization plus heterodimerization with MIG12. Other proteins known to bind to THRSP include the thyroid hormone receptor and Zac51. Heterointeractions are posited to positively influence p53-dependent gene activation. Two potential isoforms are reported. One contains a seven aa extension at the C-terminus, while another shows a deletion of aa 31-64. Over aa 47-150, mouse THRSP shares 78% and 94% aa identity with human and rat THRSP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse THRSP by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse THRSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6574) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for THRSP at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
