Mouse/Rat CD34 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse/Rat CD34 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6518-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6518-SP
别名	CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1
反应种属	Mouse/Rat
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse and rat CD34 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant porcine CD34 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	947 (Human); 12490 (Mouse); 305081 (Rat); 397160 (Porcine); 415130 (Canine)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse CD34 Thr35-Thr287 Accession # Q64314
生物活性	Mouse, Rat
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Mouse and rat CD34 is a 80 -100 kDa member of the CD34/podocalyxin family of molecules. It is a sialomucin type glycoprotein, and presents carbohydrate to selectins during cell migration. CD34 is found on mast cells, eosinophils, vascular endothelial cells, CD34⁺ stem cells and renal mesangial cells. Mature mouse CD34 is a 348 amino acid (aa) type I transmembrane protein (aa 35-382). It contains a 253 aa extracellular region (aa 35-287), and a 74 aa cytoplasmic domain that undergoes Tyr phosphorylation. Mouse and rat CD34 also is likely to undergo membrane cleavage that will generate a 54 kDa soluble form. Over aa 35-287, mouse CD34 shares 57% and 82% aa identity with human and rat Cd34, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Myeloid Leukemia B Cell Markers Cancer Stem Cell Markers Cardiac Stem Cell Markers Endothelial Cell Differentiation Markers Endothelial Cell Markers Endothelial Cell Markers and Adhesion Molecules in Angiogenesis Endothelial Progenitor and Endothelial Cell Markers Endothelial Progenitor Cell Markers Group 1 ILCs Hemangioblasts Hematopoietic Progenitor Cell Markers Hematopoietic Stem Cell Markers Human CD Marker Products ICC / IHC Images: CD34 Leukocyte Receptors/Markers in Allergy and Asthma Lymphoid Lineage Markers Muscle Stem Cell Markers Myeloid Lineage Markers Myeloid-derived Suppressor Cells (MDSC) Myogenesis Markers Myogenic Precursor Markers Regulatory T Cells (Tregs) Skin Stem Cell Markers

参考图片

Detection of Mouse and Rat CD34 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD34 at approximately 85‑90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Detection of CD34 in bEnd.3 Mouse Cell Line by Flow Cytometry. bEnd.3 mouse endothelioma cell line was stained with Mouse CD34 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6518, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).