| 货号 | AF6480-SP |
| 别名 | antigen CD66; BGP1; BGP-1; BGP1BGPBiliary glycoprotein 1; BGPI; Biliary Glycoprotein 1; biliary glycoprotein adhesion molecule; carcinoembryonic antigen-related cell adhesion molecule 1 (biliaryglycoprotein); carcinoembryonic antigen-related cell adhesion molecule 1; CD66a antigen; CD66a; Cea-1; Hv-1; Hv-2; MHVR | 全称 | Carcinoembryonic Antigen-related Cell Adhesion Molecule 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse CEACAM-1/CD66a in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 634 (Human); 26365 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CEACAM-1/CD66a Met1-Gly428 Accession # P31809 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | CEACAM-1 (Carcinoembryonic antigen-related cell adhesion molecule 1; also BGP-1, CD66a and MHVR1) is a 110-120 kDa member of the CEACAM subfamily, CEA family of proteins. It has a wide expression pattern, being found on neutrophils, dendritic cells, endothelial cells, colonic epithelium and hepatocytes. It mediates cell adhesion, and appears to regulate insulin levels and signaling by interacting with the insulin receptor. It also demonstrates proangiogenic effects by inducing endothelial cells to proliferate and form capillary-like tubules. Finally, CEACAM-1 is a known receptor for mouse hepatitis virus. Mature mouse CEACAM-1 is a 487 amino acid (aa) type I transmembrane glycoprotein. Its contains a 394 aa extracellular region (aa 35-428) that shows one V-type (aa 35-142) and three C2-type |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse CEACAM‑1/CD66a by Western Blot. Western blot shows lysates of mouse liver tissue and mouse small intestine tissue. PVDF Membrane was probed with 0.1 µg/mL of Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CEACAM‑1/CD66a between approximately 110 and 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of CEACAM-1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126) and Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A). | |
CEACAM‑1/CD66a in Mouse Liver. CEACAM‑1/CD66a was detected in perfusion fixed frozen sections of mouse liver using Sheep Anti-Mouse CEACAM‑1/CD66a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6480) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells in bile canaliculi. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
