| 货号 | AF6397-SP |
| 别名 | AAG13; Aging-associated gene 13 protein; aging-associated protein 13; DT1P1A7; MGC17528; protein S100-A16; Protein S100-F; S100 calcium binding protein A16; S100F; S100FS100 calcium-binding protein A16 | 全称 | S100 Calcium Binding Protein A16 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse S100A16 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human S100A16 is observed, and less than 1% cross-reactivity with recombinant mouse S100A4, recombinant human (rh) S100A2, and rhS100A10 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 140576 (Human); 67860 (Mouse); 361991 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E.coli-derived recombinant mouse S100A16 Ala2-Ser124 Accession # NP_080692 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Mouse S100A16 (S100 calcium-binding protein A16; also S100Fand AAG13) is a 15-18 kDa member of the S100 family of calcium-bindingproteins. It is found in both cytoplasm (whenCa is high) and nucleus (when Ca is low), and its expression appears to be restrictedto astrocytes found in select areas of the brain. S100A16 is 124 amino acids (aa) in length. Based on human,it will contain an N-terminal non-Ca-binding EF-hand domain (aa 12-47), aC-terminal Ca-binding EF-hand domain (aa 54-89), and a putative FGF-1 bindingsite (aa 91-94). There is one potentialmyristoylation site (aa 54-59) and a phosphorylation site at Ser124. S100A16 will homodimerize, and perhaps formhigher order oligomers. Full length mouseS100A16 shares 95% aa identity with rat S100A16. Relative to human S100A16, mouse S100A16possesses a 21 aa Glu/Gln-rich insertion after Glu98 that reduces the shared aahomology between mouse and human S100A16 to 72% aa identity. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse S100A16 by Western Blot. Western blot shows lysates of mouse brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Mouse S100A16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6397) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for S100A16 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
