Mouse AgRP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse AgRP Affinity Purified Polyclonal Ab (25 UG)

货号： AF634-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF634-SP
别名	agouti related protein homolog (mouse); agouti-related protein; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2
全称	Agouti-related Protein
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse AgRP in direct ELISAs and Western blots. In direct ELISAs, less than 25% cross-reactivity with recombinant human AgRP is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	181 (Human); 11604 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. QRFP-Deficient Mice Are Hypophagic, Lean, Hypoactive and Exhibit Increased Anxiety-Like Behavior PLoS ONE, 2016;11(11):e0164716. Species: Mouse Sample Type: Whole Tissue Application: IF
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse AgRP/ART Val25-Thr131 Accession # P56473
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuroprotein that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) action on MC-3 and MC-4 receptors (1-4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature mouse AgRP is a 111 amino acid (aa) polypeptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Within the C-terminal region, mouse AgRP shares 80% and 90% aa sequence identity with human and rat AgRP, respectively. It also shares 44% aa sequence identity with Agouti. As with Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP, however, is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones via a mechanism that does not involve melanocortin receptors (14).
运输条件	Blue Ice
存放说明	4℃
参考文献	Martin, N.M. et al. (2006) Peptides 27:333. Fan, W. et al. (1997) Nature 385:165. Ollmann, M.M. et al. (1997) Science 278:135. Arora, S. and Anubhuti (2006) Neuropeptides 40:375. Shutter, J.R. et al. (1997) Genes Dev. 11:593. Kiefer, L.L. et al. (1998) Biochemistry 37:991. Jackson, P.J. et al. (2002) Biochemistry 41:7565. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629. Breit, A. et al. (2006) J. Biol. Chem. 281:37447. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921. Small, C.K. et al. (2001) Diabetes 50:248. Vink, T. et al. (2001) Mol. Psychiatry 6:325. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265-266:108.

参考图片

AgRP/ART in Mouse Brain. AgRP/ART was detected in perfusion fixed frozen sections of mouse brain using Goat Anti-Mouse AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF634) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.