| 货号 | AF6325-SP |
| 别名 | BMP-6; bone morphogenetic protein 6; vegetal related growth factor (TGFB-related); vegetal-related (TGFB related) cytokine; VG-1-R; VG-1-related protein; Vg1-related sequence; VGR; VGR1; Vgr-1 | 全称 | Bone Morphogenetic Protein 6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects recombinant human and mouse BMP-6 in direct ELISAs and detects mouse BMP-6 in Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) BMP-5 and rmBMP-7 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 654 (Human); 12161 (Mouse); 25644 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse BMP-6 Ser372-His510 Accession # P20722 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Bone Morphogenetic Protein 6 (BMP‑6), also known as Vgr-1, is one of at least 15 structurally and functionally related BMPs which are members of the transforming growth factor beta (TGF-beta ) superfamily. Mouse BMP‑6 is synthesized as a 510 amino acid (aa) precursor protein that is cleaved at the dibasic cleavage site (RxxR) to release the 18 kDa C‑terminal mature protein. Biologically active BMP‑6 consists of a disulfide-linked homodimer of the mature proteins (1, 2). Mature mouse BMP‑6 shares 96% and 98% aa sequence identity with human and rat BMP‑6, respectively. Cellular responses to BMP‑6 are mediated by hetero-oligomeric complexes of type I (Activin RIA/ALK-2 and BMPR-IA/ALK-3) and type II (Activin RIIA and BMPR-II) serine/threonine kinase receptors (3‑5). Glycosylation of BMP‑6 is required for its interaction with Activin RIA (6). BMP‑6 induces the expression of Noggin and is subsequently antagonized by Noggin (7, 8). BMP‑6 induces a wide range of cellular responses. It promotes osteoblast differentiation from mesenchymal stem cells (5), chondrocyte maturation (9), Ang II-induced aldosterone production in the adrenal cortex (3), hormone production and responsiveness in ovarian granulosa cells (10), iNOS and TNF-alpha production in macrophages (4), the cell death of B cells (8), and neurite outgrowth (11). BMP‑6 expression is induced in astrocytes surrounding sites of brain injury where it functions as a neuroprotectant (11, 12). BMP‑6 is upregulated during prostate cancer progression and promotes tumor cell metastasis to bone (13). Through interactions with the BMP coreceptor RGM‑C/Hemojuvelin, BMP‑6 plays an important role in iron homeostasis by promoting Hepcidin expression and preventing serum iron overload (14, 15). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse BMP‑6 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with mouse BMP‑6. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse BMP‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). For additional reference, Recombinant Mouse BMP‑6 (Catalog # 6325-BM) (10ng/lane) was included. A specific band was detected for BMP‑6 at approximately 20kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
BMP‑6 in Mouse Embryo. BMP‑6 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse BMP‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6325) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to skeletal muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
