| 货号 | AF6265-SP |
| 别名 | F2771; RIBP; SCAP; SH2 domain containing 2A; SH2 domain protein 2A; SH2 domain-containing adapter protein; T cell specific adapter protein TSAd; T cell-specific adapter protein; T lymphocyte specific adaptor protein; TSAd; TSADSH2 domain-containing protein 2A; VEGF receptor-associated protein; VRAP |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse and rat RIBP/SH2D2A in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9047 (Human); 27371 (Mouse); 310688 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse RIBP/SH2D2A Ile299-Ser374 Accession # Q9QXK9 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.5 mg/mL in sterile PBS. |
| 背景 | RIBP (Rlk/Itk‑binding protein; also SH2D2A, LAD/Lck‑associated adapter protein, MEKK2‑binding protein and TSAd) is a 45‑47 kDa cytoplasmic adaptor protein. It is found in B cells, thymocytes, NK cells and T cells, and appears to be necessary for TCR‑initiated proliferation, plus IL‑2 and IFN‑ gamma secretion. This is likely to be a function of its association with MEKK2 near the T cell receptor interaction site. Mouse RIBP is 374 amino acids (aa) in length and contains one SH2 domain (aa 116‑207), plus a Pro‑rich region (aa 267‑308) that contains an SH3‑binding motif (aa 267‑273). Phosphorylation may occur on Ser237, Ser316 and Tyr325. There is one alternative splice form termed LAD that shows a deletion of aa 254‑261. Over aa 299‑374, mouse RIBP shares 79% and 63% aa identity with rat and the presumed human RIBP ortholog (termed TSAd/SH2D2A), respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat RIBP/SH2D2A by Western Blot. Western blot shows lysates of CTLL‑2 mouse cytotoxic T cell line and Nb2‑11 rat lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat RIBP/SH2D2A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6265) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RIBP/SH2D2A at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
