| 货号 | AF6239-SP |
| 别名 | KIAA1745sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4B; MGC131831; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4B; SEMA4B; SEMAC; semaphorin-4B; SemC |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Semaphorin 4B in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human Semaphorin 4B is observed and less than 2% cross‑reactivity with recombinant mouse (rm) Semaphorin 4A and rmSemaphorin 4C is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10509 (Human); 20352 (Mouse); 293042 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4B Leu31-Glu703 Accession # Q62179 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Semaphorin 4B (Sema4B; also SemaC) is a 95‑100 kDa, class IV member of the semaphorin family of proteins. It is expressed in neurons, and following PSD-95 induced clustering, participates in the formation or functioning of glutamatergic synapses. Mature mouse Sema4B is a type I transmembrane glycoprotein that is 793 amino acids (aa) in length. It contains a 673 aa extracellular region (aa 31‑703) that is characterized by one Sema domain (aa 57‑492), a PSI region (aa 512‑582), and an Ig‑like C2‑type domain (aa 589‑649). There are three phosphoserines in the cytoplasmic tail. Over aa 31‑703, mouse Sema4B shares 86% and 95% aa identity with human and rat Sema4B, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Semaphorin 4B by Western Blot. Western blot shows lysates of mouse pancreas tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6239) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4B at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
