Mouse LRRC32/GARP Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse LRRC32/GARP Affinity Purified Polyclonal Ab (25 UG)

货号： AF6229-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6229-SP
别名	D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32
全称	Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) LRRC32 is observed and less than 5% cross-reactivity with rhLRRC3, rhLRRC4, and rhLRRC4B is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2615 (Human); 434215 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP Ile18-Asn628 Accession # NP_001106850
生物活性	Mouse
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	Leucine-rich repeat protein 32 (LRRC32), also known as GARP (glycoprotein A repetitions predominant), is an 80 kDa type I transmembrane glycoprotein (1). Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain (2-4). Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is widely expressed during embryogenesis and on adult platelets (4, 5). Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg) (6-10). LRRC32 expression promotes the acquisition of a Treg phenotype including reduced cellular proliferation, reduced cytokine secretion, and the capacity to suppress the proliferation of naïve T cells (6-8). LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and tethers latent TGF-beta on the surface of activated Treg cells (9, 10). The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation (11).
运输条件	Blue Ice
存放说明	4℃
参考文献	Battaglia, M. and M.G. Roncarolo (2009) Eur. J. Immunol. 39:3296. Ollendorff, V. et al. (1994) Cell Growth Differ. 5:213. Bella, J. et al. (2008) Cell Mol Life Sci. 65:2307. Roubin, R. et al. (1996) Int. J. Dev. Biol. 40:545. Macaulay, I.C. et al. (2007) Blood 109:3260. Wang, R. et al. (2008) PloS ONE 3:e2705. Wang, R. et al. (2009) Proc. Natl. Acad. Sci. 106:13439. Probst-Kepper, M. et al. (2009) J. Cell. Mol. Med. 13:3343. Tran, D.Q. et al. (2009) Proc. Natl. Acad. Sci. 106:13445. Stockis, J. et al. (2009) Eur. J. Immunol. 39:3315. Vignali, D.A. et al. (2008) Nat. Rev. Immunol. 8:523.

参考图片

Detection of Mouse LRRC32/GARP by Western Blot. Western blot shows lysates of mouse spleen non-B cells. PVDF Membrane was probed with 0.1 µg/mL of Sheep Anti-Mouse LRRC32/GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRC32/GARP at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

LRRC32/GARP in bEnd.3 Mouse Cell Line. LRRC32/GARP was detected in immersion fixed bEnd.3 mouse endothelioma cell line using Sheep Anti-Mouse LRRC32/
GARP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6229) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.