Mouse/Rat NCAM-1/CD56 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse/Rat NCAM-1/CD56 Affinity Purified Polyclonal Ab (25 UG)

货号： AF6070-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF6070-SP
别名	CD56 / NCAM-1; CD56 antigen; CD56; MSK39; N-CAM-1; NCAM-1; NCAMantigen recognized by monoclonal 5.1H11; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM
全称	Neural Cell Adhesion Molecule
反应种属	Mouse/Rat
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse and rat NCAM-1/CD56 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human NCAM-1 is observed.
使用方法	Western Blot: 1 µg/mL
来源	Sterile PBS to a final concentration of 0.2 mg/mL.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4684 (Human); 17967 (Mouse); 24586 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse NCAM-1/CD56 Leu20-Thr711 (Phe403Val) Accession # P13595
生物活性	Mouse, Rat
标记	Unconjugated
溶解方法	Sterile PBS to a final concentration of 0.2 mg/mL.
背景	NCAM-1 (Neural adhesion molecule-1; also CD56) is a 120 -190 kDa glycoprotein member of the Ig Superfamily. It is expressed on multiple cell types, both in the embryo and adult. Here, it serves as both an adhesion molecule and a receptor for multiple ligands, including as FGFR, PDGF, GDNF and agrin. On the cell surface, it is a cis-oriented homodimer that can form homodimers in-trans with other cis-homodimers. In the embryo, NCAM-1 is polysialylated (PolySia), and shows a MW of 200-220 kDa in SDS-Page. This polysialylation reduces the ability of NCAM-1 to dimerize. Mature mouse NCAM-1 is a 1096 amino acid (aa) type I transmembrane (TM) protein (aa 20-1115). It possesses a 692 aa extracellular region (aa 20-711) and a 386 aa cytoplasmic domain. The extracellular region contains five consecutive C2-type Ig-like domains (aa 20-492) followed by two FN type-III domains (aa 497-692). Multiple splice variants exist. There is a 140 kDa TM variant that shows a deletion of aa 810-1076, and a 120 kDa variant that is GPI-linked and shows a 24 aa substitution for aa 702-1115. A third potential variant contains a five aa substitution for aa 601-1115. Over aa 20-711, mouse NCAM-1 shares 99% and 95% aa identity with rat and human NCAM-1, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	Acute Myeloid Leukemia Adult Neurogenesis Cancer Biomarkers Conventional/Classical Dendritic Cells GDNF Family Group 1 ILCs Human CD Marker Products ICC / IHC Images: NCAM-1/CD56 Immunoglobulin Superfamily CAMs Inflammatory/Monocyte-derived Dendritic Cells Isolation, Culture, and Identification of Natural Killer Cells Langerhans and Dermal Dendritic Cells Lymphoid Lineage Markers Mesenchymal Stem Cell Markers Multiple Myeloma Myogenesis Markers Myogenic Precursor Markers Natural Killer T (NKT) Cells NCAM as a Novel GDNF Family Receptor NCR+ILC3 NCR-ILC3 Neural Progenitor Cell Markers Plasmacytoid Dendritic Cells Skeletal Muscle Markers Synaptic Adhesion Molecules Synaptic Proteins

参考图片

Detection of Rat NCAM‑1/CD56 by Western Blot. Western blot shows lysates of rat hippocampal embryonic neurons. PVDF Membrane was probed with 1 µg/mL of Mouse NCAM‑1/CD56 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCAM‑1/CD56 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.