| 货号 | AF603-SP |
| 别名 | Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Ephrin-A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Ephrin-A1, recombinant human (rh) Ephrin-A3, rmEphrin-A4, rhEphrin-A5, rmEphrin-B1, rmEphrin-B2, and rhEphrin-B3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2) to immobilized Recombinant Rat EphA5 Fc Chimera (Catalog # 541-A5) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding. Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1943 (Human); 13637 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A2 Met1-Asn184 Accession # P52801 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Ephrin-A2, also known as ELF-1, Cek7-L and LERK-6 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A2 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of human and mouse Ephrin-A2 share 93% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression.(3) It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Ephrin‑A2 in Rat Hippocampal Neurons. Ephrin‑A2 was detected in immersion fixed rat hippocampal neurons using 10 µg/mL Goat Anti-Mouse Ephrin‑A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF603) for 3 hours at room temperature. Neurons were stained (green) and glial cells were labeled by using anti-GFAP antibodies (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
