| 货号 | AF5997-SP |
| 别名 | CC chemokine receptor 8; C-C CKR-8; CC-chemokine receptor chemr1; CC-CKR-8; CCR8; CCR-8; CD198; CDw198 antigen; CDw198; chemokine (C-C motif) receptor 8; chemokine (C-C) receptor 8; chemokine (C-C) receptor-like 2; Chemokine receptor-like 1; ChemR1; CKRL1; CKR-L1; CKRL1MGC129966; CMKBR8MGC129973; CMKBRL2C-C chemokine receptor type 8; CY6; GPRCL1; GPRCY6; GPR-CY6; TER-1; TER1CC chemokine receptor CHEMR1 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(0.5 µg/mL) |
| 目标/特异性 | Detects mouse and rat CCR8 in direct ELISAs and Western blots. In direct ELISAs, less than 2% cross‑reactivity with recombinant mouse (rm) CCR1, rmCCR4, and rmCCR5 is observed. |
| 使用方法 | Western Blot: 0.5 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1237 (Human); 12776 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse CCR8 Met1-Met33, Asn92-Lys105, Tyr170-Ile200, His262-Leu278 Accession # P56484 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CCR8 (C-C chemokine receptor 8; also CD198) is a 41‑43 kDa member of the GPCR #1 family of transmembrane proteins. Mouse CCR8 is expressed on vascular smooth muscle cells, monocytes, eosinophils, peritoneal macrophages, thymocytes, CD8+ T cells, Langerhans cells and neurons. CCL1/TCA3 and vMIP-1 are known agonists for CCR8. Mouse CCR8 is a 7-transmembrane protein that is 353 amino acids (aa) in length. It contains a 33 aa N-terminal extracellular domain plus a 50 aa C-terminal cytoplasmic tail. In mouse, CCR8 is N- and possibly O‑glycosylated, and known to be sulfated on Tyr14 and 15. The unusual nature of these posttranslational modifications may lead to anomalous migration in SDS-PAGE. There are two potential isoforms, one that shows a deletion of aa 103‑163, and another that shows a Met substitution for aa 125‑166. Over aa sequences 1‑33 and 92‑105 collectively, mouse CCR8 shares 64% and 85% aa identity with human and rat CCR8, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse and Rat CCR8 by Western Blot. Western blot shows lysates of mouse thymus tissue and rat thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse/Rat CCR8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5997) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CCR8 at approximately 41-43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
