| 货号 | AF5975-SP |
| 别名 | CD205; Ly75 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse DEC-205/CD205 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human DEC-205 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4065 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse DEC-205/CD205 Cys216-Pro503 Accession # Q60767 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DEC-205 (Dendritic and thymic EpithelialCell-205; also Ly-75 and CD205) is a 200-205 kDa member of the mannose receptor family of molecules. In mouse, it is widely expressed, being found on B cells, Langerhans cells, bone marrow-derived dendritic cells, neutrophils, plus respiratory, intestinal, and thymic cortical epithelium. DEC-205 serves as a recognition/endocytic receptor for dying cells, and likely participates in the induction of self-tolerance. Mature mouse DEC-205 is a 1696 amino acid (aa) type I transmembrane glycoprotein (aa 28-1723). It contains a 1640 aa extracellular domain (ECD) (aa 28-1667) and a 31 aa cytoplasmic tail. The ECD is highly modular, containing a ricin B-type lectin domain (aa 33-182), a FN type II domain (aa 164-211) and nine sequential C-type lectin domains (aa 225-1662). There are at least two potential isoform variants. One shows a two aa substitution for aa 866-1723, and another shows a 64 aa substitution for aa 734-1723. Over aa 216-503, mouse DEC-205 shares 87% and 79% aa identity with rat and human DEC-205, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse DEC‑205/CD205 by Western Blot. Western blot shows lysates of JAWSII mouse immature dendritic cell line and mouse thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DEC‑205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DEC‑205/CD205 at approximately 205 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
