Mouse IL-21 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-21 Affinity Purified Polyclonal Ab (25 UG)

货号： AF594-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF594-SP
别名	IL-21Za11interleukin-21; interleukin 21; interleukin-21 isoform
全称	Interleukin 21
反应种属	Mouse
应用	Western Blot,Immunocytochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse IL-21 in ELISAs and Western blots. In sandwich ELISAs, less than 0.5% cross-reactivity with recombinant mouse (rm) IL‑2, rmIL‑4, rmIL-15, and recombinant human IL-21 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize IL‑21-induced proliferation in the N1186 human T cell line. The Neutralization Dose (ND₅₀) is typically 5-15 µg/mL in the presence of 250 ng/mL Recombinant Mouse IL‑21.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	59067 (Human); 60505 (Mouse); 365769 (Rat); 442935 (Canine); 100344172 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Targeting the binding interface on a shared receptor subunit of a cytokine family enables the inhibition of multiple member cytokines with selectable target spectrum. Authors: Nata T, Basheer A, Cocchi F, van Besien R, Massoud R, Jacobson S, Azimi N, Tagaya Y J Biol Chem, 2015;290(37):22338-51. Species: Mouse Sample Type: Whole Cells Application: Neut Interleukin-21 is critically required in autoimmune and allogeneic responses to islet tissue in murine models. Authors: McGuire HM, Walters S, Vogelzang A Diabetes, 2011;60(3):867-75. Species: Mouse Sample Type: In Vivo Application: In vivo STAT3-dependent IL-21 production from T helper cells regulates hematopoietic progenitor cell homeostasis. Authors: Kaplan MH, Glosson NL, Stritesky GL, Yeh N, Kinzfogl J, Rohrabaugh SL, Goswami R, Pham D, Levy DE, Brutkiewicz RR, Blum JS, Cooper S, Hangoc G, Broxmeyer HE Blood, 2011;117(23):6198-201. Species: Mouse Sample Type: In Vivo Application: In vivo IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE). Authors: Rynda A, Maddaloni M, Ochoa-Reparaz J, Callis G, Pascual DW PLoS ONE, 2010;5(1):e8720. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Cytokine-secreting follicular T cells shape the antibody repertoire. Authors: Reinhardt RL, Liang HE, Locksley RM Nat. Immunol., 2009;10(4):385-93. Species: Mouse Sample Type: Whole Cells Application: ELISpot Development Tumor-specific Th17-polarized cells eradicate large established melanoma. Authors: Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Blood, 2008;112(2):362-73. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development IL-21 is produced by Th17 cells and drives IL-17 production in a STAT3-dependent manner. Authors: Wei L, Laurence A, Elias KM, OShea JJ J. Biol. Chem., 2007;282(48):34605-10. Species: Mouse Sample Type: Whole Cells Application: Neut IL-21 induces tumor rejection by specific CTL and IFN-gamma-dependent CXC chemokines in syngeneic mice. Authors: Di Carlo E, Comes A, Orengo AM, Rosso O, Meazza R, Musiani P, Colombo MP, Ferrini S J. Immunol., 2004;172(3):1540-7. Species: Mouse Sample Type: Cell Lysates Application: WB Expression and one-step purification of bovine interleukin-21 (IL-21) in silkworms using a hybrid baculovirus expression system. Authors: Muneta Y, Nagaya H, Minagawa Y, Enomoto C, Matsumoto S, Mori Y Biotechnol. Lett., 2004;26(18):1453-8. Species: Bovine Sample Type: recombinant IL-21 Application: WB IL-21 induces the functional maturation of murine NK cells. Authors: Brady J, Hayakawa Y, Smyth MJ, Nutt SL J. Immunol., 2004;172(4):2048-58. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL‑21 (R&D Systems, Catalog # 594-ML) Pro25-Ser146 Accession # Q9ES17
内毒素水平	<0.30 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin-21 (IL-21) and its receptor appear to play important roles in the regulation of the immune system. IL-21 is most related to IL-2, IL-4, and IL-15. IL-21 R, also called NILR (novel interleukin receptor), is a type I cytokine receptor with 4 conserved cysteine residues and an extracellular WSXWS motif. It is most closely related to IL-2 R beta and IL-4 R alpha. Mouse IL-21 is a 146 amino acid (aa) residue protein with a 24 aa signal peptide. Mouse and human IL-21 share 57% amino acid sequence identity. IL‑21 is expressed by activated T cells. Although not fully elucidated, the IL-2 R gamma ( gamma _c) chain appears to play a role in IL-21 R signaling. The IL‑21/IL‑21 R interaction appear to play important roles in B and T cell proliferation after antigen stimulation and NK cell maturation.
运输条件	Blue Ice
存放说明	4℃
参考文献	Parrish-Novak,et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) PNAS 97:11439. Dumoutier, L. et al. (2000) Proc. Natl. Acad. Sci. USA 97:10144. Asao, H. et al. (2001) J. Immunol. 167:1.

参考图片

Cell Proliferation Induced by IL‑21 and Neutralization by Mouse IL‑21 Antibody. Recombinant Mouse IL‑21 (Catalog # 594-ML) stimulates proliferation in the N1186 human T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑21 (250 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594). The ND₅₀ is typically 5-15 µg/mL.

IL‑21 in Mouse Splenocytes. IL‑21 was detected in immersion fixed concanavalin A-stimulated mouse splenocytes using 15 µg/mL Mouse IL‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF594) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.