| 货号 | AF5919-SP |
| 别名 | Activity-dependent neuroprotective protein; activity-dependent neuroprotector homeobox; activity-dependent neuroprotector; ADNP homeobox 1; ADNP1; ADNP1KIAA0784activity-dependent neuroprotector homeobox protein | 全称 | Activity-dependent Neuroprotective Protein |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse and rat ADNP in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human ADNP is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23394 (Human); 11538 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Novel evolutionary-conserved role for the activity-dependent neuroprotective protein (ADNP) family that is important for erythropoiesis. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse ADNP Leu941-Ala1102 Accession # Q9H2P0 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ADNP (Activity-dependent neuroprotective protein) is an ADNF-related factor found in astrocytes and microvascular endothelial cells. Although its predicted MW is 124 kDa, it runs anomalously at 148‑150 kDa in SDS-PAGE. ADNP is inducible by VIP in astrocytes, and serves as a neuroprotector, both outside and inside the cell. Extracellularly, ADNP may protect synapse activity; intracellularly, ADNP interacts with tubulin, promoting microtubule assembly, and within the nucleus, it contributes to the SWI/SNF chromatin remodeling complex. Mouse ADNP is 1108 amino acids (aa) in length. It contains a neuroprotective peptide/NAP (aa 354‑361) embedded between nine C2H2-type Zn finger domains (aa 76‑685), a DNA-binding homeobox region (aa 752‑813) and a Leu-rich NLS that overlaps a protein internalization sequence (aa 787‑812). There are alternative start sites at Met229 and Met281. Over aa 947‑1108, mouse ADNP shares 98% and 93% aa identity with rat and human ADNP, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse/Rat ADNP by Western Blot. Western blot shows lysates of embryonic rat cortical neuron/ glial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ADNP at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ADNP in RAW 264.7 Mouse Cell Line. ADNP was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
ADNP in Mouse Brain. ADNP was detected in perfusion fixed frozen sections of mouse brain (cerebellum) using Goat Anti-Mouse/Rat ADNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5919) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Specific staining was localized to Purkinje cells and neurons in molecular cell layer. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
