| 货号 | AF5917-SP |
| 别名 | BMP-15; bone morphogenetic protein 15; GDF-9B; GDF9BODG2; Growth/differentiation factor 9B; POF4 | 全称 | Bone Morphogenetic Protein 15 |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse and rat BMP-15/GDF-9B in Western blots and recombinant mouse in direct ELISAs. In direct ELISAs, approximately 5% cross‑reactivity with recombinant human (rh) BMP‑15 is observed and less than 1% cross‑reactivity with rhBMP-3 and rhBMP-10 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9210 (Human); 12155 (Mouse); 59302 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse BMP-15/GDF-9B Gln268-Arg392 Accession # Q9Z0L4 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | BMP-15 (Bone morphogenetic protein 15; also GDF-9B) is a 24-26 kDa member of the TGF-beta superfamily of proteins. It is expressed by late primary follicle oocytes, where it promotes the transition of preantral granulosa cells to cumulus cells, and later the expansion of cumulus cells. Mouse BMP-15 proprecursor is a 50-55 kDa, 367 amino acid (aa) glycoprotein. It is proteolytically cleaved to generate a 40 kDa prosegment (aa 26-267) plus a 24 kDa, 124 aa mature region (aa 268-392) that may be phosphorylated (on Ser7 of the mature molecule) and/or glycosylated. Secreted BMP-15 does not occur as a mature homodimer, but it does exist as a mature monomer, an uncleaved proprecursor, or as a noncovalent heterodimer composed of a cleaved mature region and its prosegment. The heterodimer may also form an oligomer. The BMP-15 prosegment reportedly forms a noncovalent heterodimer with 20 kDa mature GDF-9. Mature mouse BMP-15 shares 70% and 91% aa identity with human and rat BMP-15, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Rat BMP‑15/GDF‑9B by Western Blot. Western blot shows lysates of rat ovary tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat BMP‑15/GDF‑9B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5917) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BMP‑15/GDF‑9B proprecursor at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
