| 货号 | AF5915-SP |
| 别名 | CRP-ductin; deleted in malignant brain tumors 1 protein; deleted in malignant brain tumors 1; Ebnerin; FLJ61058; Glycoprotein 340; GP340; gp-340; GP340muclin; Hensin; MGC164738; Muclin; SAG; Salivary agglutinin; Surfactant pulmonary-associated D-binding protein; Vomeroglandin | 全称 | Deleted in Malignant Brain Tumors 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse DMBT1 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1755 (Human); 12945 (Mouse); 170568 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse DMBT1 Trp215-Gly420 Accession # Q60997 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DMBT1 (Deleted in malignant brain tumor-1; also MUCLIN, Hensin, CRP-Ductin, and gp300/350) is a 230‑350 kDa member of the DMBT1 family of molecules. It is expressed by select cell types, including macrophages, intestinal epithelium, and pancreatic acinar cells. DMBT1 is both transmembrane (TM) and secreted, and possesses multiple activities that may be predicated on multimerization. It drives epithelial differentiation, binds Gm+ and Gm- bacteria, and associates with cytoskeletal actin. Mouse DMBT1 is a type I TM glycoprotein 2085 amino acids (aa) in length. Its extracellular region contains eight SRCR repeats (aa 31‑980, plus 1510‑1610), five CUB domains (aa 1023‑1490, plus 1633‑1742), and a ZP segment (aa 1751‑1999). Multiple splice variants exist. There are deletions of aa 584‑733, 397‑534, 593‑731 and, 2032‑2085 (within the TM segment), plus an insertion of 11 aa after Asp29. Over aa 215‑420, mouse DMBT1 shares 75% aa identity with human DMBT1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse DMBT1 by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse DMBT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5915) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for DMBT1 at approximately 240 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
