| 货号 | AF5804-SP |
| 别名 | Deubiquitinating enzyme 2; EC 3.1.2.15; EC 3.4.19.12,41 kDa ubiquitin-specific protease; ubiquitin specific peptidase 2; ubiquitin specific protease 12; ubiquitin specific protease 2; ubiquitin specific protease 9; ubiquitin thioesterase 2; Ubiquitin thiolesterase 2; Ubiquitin-specific-processing protease 2; UBP41; UBP41ubiquitin carboxyl-terminal hydrolase 2; USP9 | 全称 | Ubiquitin-specific Protease 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse USP2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human USP2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 9099 (Human); 53376 (Mouse); 115771 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse USP2 isoform 2 Lys47-Met396 Accession # NP_932759 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | USP2 (Ubiquitin-specific-processing protease 2; also Ubiquitin carboxy-terminal hydrolase 2) is a cytosolic member of the peptidase C19 family of enzymes. It is widely expressed and serves to deubiquitinate select target substrates. USP2 effects are determined by its targets. In cells where it increases Mdm2, p53 is degraded and apoptosis is blocked. In cells where it increases Itch concentration, Flip activity is interrupted and apoptosis is promoted. USP2 is reported to form homo‑oligomers. There are at least two isoform variants. Each is approximately 70 kDa in size, with one showing a 266 aa, and another a 272 aa substitution for aa 1‑49. Over aa 47‑396, mouse USP2 shares 96% and 99% aa identity with human and rat USP2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse USP2 by Western Blot. Western blot shows lysates of mouse liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse USP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5804) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for USP2 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
