Mouse alpha 2-Macroglobulin Affinity Purified Polyclonal Ab (25 UG)
货号：
AF5798-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF5798-SP |
| 别名 | A2M; alpha-2-M; alpha-2-macroglobulin; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; CPAMD5; CPAMD5DKFZp779B086; FWP007; S863-7 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL)
|
| 目标/特异性 | Detects mouse alpha 2-Macroglobulin in direct ELISAs and Western blots. In direct ELISA, less than 2% cross-reactivity with human alpha 2‑Macroglobulin, recombinant rat (rr) alpha 2-Macroglobulin, and rr beta 2-Macroglobulin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse serum-derived alpha 2-Macroglobulin |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | alpha2-Macroglobulin (alpha 2 macroglobulin; also A2M and MAM) is a 185 kDa member of the protease inhibitor I39 family of proteins. It is secreted by the liver, circulates at a constant level in the blood, binds multiple growth factors, inhibits a wide variety of proteinases via steric trapping, is cleared by cell surface receptors and likely exists as a tetramer. Mature mouse A2M is 1471 amino acids (aa) in length. It is cleaved between Arg1239 and Asp1240 to generate two subunits, one that is 165 kDa in size and 1215 aa in length and a second that is 35 kDa in size and 256 aa in length. The protease inhibitor region (aa 686-744) is on the large subunit, while the receptor binding segment (aa 1395-1482) is on the smaller subunit. A2M has a counterpart gene termed A2M-P that is associated with pregnancy. The A2M-P molecule shares 56% aa identity with mouse A2M. Notably, mouse A2M-P is closer in aa sequence to human A2M (72%) and rat A2M (91%) than mouse A2M is to human A2M (59%) and rat A2M (56%). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Phase Proteins
- APP Cleavage and Amyloid-beta Degradation
- Aspartic Proteases and Regulators
- Cell Structure and Signaling Molecules
- Coagulation
- Cysteine Proteases and Regulators
- FGF Family
- ICC / IHC Images: alpha 2-Macroglobulin
- Interactive Pathway: Blood Coagulation Signaling Pathways
- Metalloproteases and Regulators
- MMPs, TIMPs and Related Molecules
- Serine Proteases and Regulators
|
参考图片Detection of Mouse alpha 2-Macroglobulin by Western Blot. Western blot shows samples of purified alpha 2-Macroglobulin from mouse sera. PVDF membrane was probed with 0.1 µg/mL Sheep Anti-Mouse alpha 2-Macroglobulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5798) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for alpha 2-Macroglobulin was detected at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
