| 货号 | AF575-SP |
| 别名 | CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse E-Selectin/CD62E in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human E-Selectin, recombinant mouse (rm) L-Selectin, and rmP-Selectin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6401 (Human); 20339 (Mouse); 25544 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice. | |
| 纯化方式 | Antigen Affinity-purified from egg yolks |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse E-Selectin/CD62E Trp22-Pro557 Accession # Q3U5F6 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, or CD62E), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha, and demonstrates peak expression at 4 hours, and decay at 24 hours, in response to activation. E-Selectin ligands, expressed on neutrophils, monocytes, and a subset of memory T cells, are sialylated, fucosylated molecules which bind to the lectin domain of E-Selectin. Immunocytochemical techniques have demonstrated the expression of E‑Selectin on healthy and diseased tissue. The human and mouse E-Selectin mediates the attachment of flowing leukocytes to the blood vessel wall during inflammation by binding to E-Selectin ligands on leukocytes. These interactions are labile and permit leukocytes to roll along the vascular endothelium in the direction of blood flow. This initial interaction is followed by a stronger interaction involving ICAM-1 and VCAM-1 that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble E-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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E-Selectin/CD62E in Mouse Thymus. E-Selectin/CD62E was detected in perfusion fixed frozen sections of mouse thymus using Mouse E-Selectin/CD62E Antigen Affinity-purified Polyclonal Antibody (Catalog # AF575) at 1.7 µg/mL overnight at 4 °C. Tissue was stained (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to the plasma membrane of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
