| 货号 | AF569-SP |
| 别名 | EFL4; EFL-4; EFNA4; EPH-related receptor tyrosine kinase ligand 4; ephrin-A4; EPLG4MGC125826; LERK-4; LERK4FLJ57652; ligand of eph-related kinase 4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse Ephrin‑A4 in direct ELISAs and Western blots. In Western blots, approximately 35% cross‑reactivity with recombinant human (rh) Ephrin‑A4 is observed and less than 5% cross‑reactivity with rhEphrin-B3, recombinant mouse (rm) Ephrin‑A1, rmEphrin-A2, rhEphrin-A5, and rhEphrin-A3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1945 (Human); 13639 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Ephrin-A4 Met1-Gly176 Accession # O08542 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | There are two structural groups of ephrin ligands which bind to a family of receptor tyrosine kinases (Eph). A-type ephrins bind A-type receptors, which are tethered to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. B-type ephrins bind to B-type receptors, which have a single transmembrane domain and a cytoplasmic region. All ephrin ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. Only membrane bound or Fc-clustered ephrin ligands are capable of activating the receptor in vitro. Soluble monomeric ephrin ligands bind the receptor but do not induce receptor autophosphorylation and activation. In vivo, ephrin ligands and receptors display reciprocal expression. It has been found that nearly all ephrin receptors and ligands are expressed in developing and adult neural tissue. The Eph/ephrin families also appear to play a role in angiogenesis and synapse formation. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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