| 货号 | AF554-SP |
| 别名 | CD4 antigen (p55); CD4 antigen; CD4 molecule; CD4 receptor; CD4; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse CD4 in direct ELISAs and Western blots. In these formats, less than 1% cross‑reactivity with recombinant human CD4, recombinant feline CD4, and recombinant canine CD4 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Determinants in the beta and delta subunit cytoplasmic loop regulate Golgi trafficking and surface expression of the muscle acetylcholine receptor. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse CD4 Lys27-Thr394 Accession # P06332 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD4 (Cluster of Differentiation #4; also L3T4) is a 55 kDa member of the Ig Superfamily of molecules. It is expressed on a variety of hematopoietic cell types, including immature thymocytes, a number of variable phenotype single positive T cells (i.e.- CD25+; CD39+; Th1, 2 and 17 type cells), stimulated CD8+ T cells, pluripotent bone marrow stem cells, and NKT cells. Although CD4 is a standard phenotype marker, functionally, the molecule is known to bind to APC MHC class II, and to associate with adjacent CD3 molecules to link p56lck with the lymphocyte TCR:CD3 complex. Mature mouse CD4 is a 431 amino acid (aa) type I transmembrane glycoprotein. It contains a 368 aa extracellular region (aa 27-394) plus a 40 aa cytoplasmic domain (aa 418-457). The extracellular region contains one V-type (aa 27-128) and three C2-type Ig-like domains (aa 129-374). There is one alternative start site at Met241 that is utilized in brain. Over aa 27-394, mouse CD4 shares 53% and 72% aa identity with human and rat CD4, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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