Mouse IL-15 R alpha Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-15 R alpha Affinity Purified Polyclonal Ab (25 UG)

货号： AF551-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF551-SP
别名	CD215 antigen; CD215; IL-15 receptor subunit alpha; IL15RA; IL-15RA; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179
全称	Interleukin 15 Receptor alpha
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse IL-15 R alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse (rm) IL-2 R beta, and rmIL-2 R gamma is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.3-1.5 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL‑15.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3601 (Human); 16169 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins. Authors: Klezovich-Benard M, Corre JP, Jusforgues-Saklani H, Fiole D, Burjek N, Tournier JN, Goossens PL PLoS Pathog., 2012;8(1):e1002481. Species: Mouse Sample Type: Whole Cells Application: Neut Paracrine and transpresentation functions of IL-15 are mediated by diverse splice versions of IL-15Ralpha in human monocytes and dendritic cells. Authors: Muller J, Waldmann T, Kruhlak M, Dubois S J Biol Chem, 2012;287(48):40328-38. Species: Mouse Sample Type: Cell Lysates Application: WB Mouse fetal and embryonic liver cells differentiate human umbilical cord blood progenitors into CD56-negative natural killer cell precursors in the absence of interleukin-15. Authors: McCullar V, Oostendorp R, Panoskaltsis-Mortari A, Yun G, Lutz CT, Wagner JE, Miller JS Exp. Hematol., 2008;36(5):598-608. Species: Mouse Sample Type: Whole Cells Application: Flow Immunological mechanisms and clinical implications of regulatory T cell deficiency in a systemic autoimmune disorder: roles of IL-2 versus IL-15. Authors: Yang CH, Tian L, Ling GS, Trendell-Smith NJ, Ma L, Lo CK, Stott DI, Liew FY, Huang FP Eur. J. Immunol., 2008;38(6):1664-76. Species: Mouse Sample Type: Whole Cells Application: Flow Combined stimulation with interleukin-18 and CpG induces murine natural killer dendritic cells to produce IFN-gamma and inhibit tumor growth. Authors: Chaudhry UI, Kingham TP, Plitas G, Katz SC, Raab JR, DeMatteo RP Cancer Res., 2006;66(21):10497-504. Species: Mouse Sample Type: Whole Cells Application: Flow Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis. Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP J. Immunol., 2004;172(3):1754-62. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha Gly33-Lys205 Accession # Q60819
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL-15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF-alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.
运输条件	Blue Ice
存放说明	4℃
参考文献	Anderson, D.M.et al. (1995) J. Biol. Chem. 270:29862. Bulfone-Paus, S.et al. (1999) FASEB 13:1575. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19. Dubois, S.et al. (1999) J. Biol. Chem. 274:26978.

参考图片

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 R alpha Antibody. Recombinant Human IL‑15 (Catalog # 247-IL) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑15 R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF551). The ND₅₀ is typically 0.3-1.5 µg/mL.