货号 | AF5500-SP |
别名 | TLT4 | 全称 | Triggering Receptor Expressed on Myeloid Cells-like 4 |
反应种属 | Mouse |
应用 | Western Blot(1 µg/mL) |
目标/特异性 | Detects mouse TREML4/TLT‑4 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) TREM1, rmTREM2b, and rmTREM3 is observed. |
使用方法 | Western Blot: 1 µg/mL |
来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
产品组分 |
供应商 | R&D Systems |
Entrez Gene IDs | 285852 (Human); 224840 (Mouse); 680862 (Rat) |
纯化方式 | Antigen Affinity-purified |
免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse TREML4/TLT‑4 Ser25-Val195 Accession # NP_001029094 |
生物活性 | Mouse |
标记 | Unconjugated |
溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
背景 | TREML4 (Triggering receptor expressed on myeloid cells-like4; also TLT4) is a 53-55 kDa member of the Ig superfamily. It is likely expressed by leukocytes, andbased on its structure, may act as an activating receptor. Mature mouse TREML4 is a type I transmembrane(TM) glycoprotein that is 235 amino acids (aa) in length. It contains a 171 aaextracellular region (aa 29-199) with a V-type Ig-like domain (aa 29-122); theTM segment contains one |
运输条件 | Blue Ice |
存放说明 | 4℃ |
Detection of Mouse TREML4/TLT‑4 by Western Blot. Western blot shows lysates of mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse TREML4/TLT‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5500) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TREML4/TLT‑4 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |