| 货号 | AF5406-SP |
| 别名 | DKFZp761K2315; heparan sulfate 6-O-sulfotransferase 3 |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 266722 (Human); 50787 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 Pro28-Trp470 Accession # NP_056635 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta -secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 in Mouse Brain. Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Anti-Mouse Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5406) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
