| 货号 | AF5375-SP |
| 别名 | CD232 antigen; CD232; plexin (semaphorin receptor); plexin C1; PLXNC1; PLXN-C1; receptor for viral semaphorin protein; receptor for virally-encoded semaphorin; VESPR; VESPRplexin-C1; Virus-encoded semaphorin protein receptor |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Plexin C1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse Plexin A1, A2, A3, recombinant human Plexin B1, B2, and D1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10154 (Human); 54712 (Mouse); 362873 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse Plexin C1 Ala35-Thr950 Accession # Q9QZC2 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Plexin C1 (also VESPR and CD232) is a 200‑210 kDa member of the plexin C subfamily, plexin family of proteins. It is a type I transmembrane glycoprotein that is found on monocytes, dendritic cells, neutrophils and embryonic neuroendocrine neurons of the hyypothalamus. Plexin C1 binds Sema7A, and its viral counterpart, vaccinia semaphorin A39R. Ligation induces actin cytoskeletal rearrangement and cell immobilization. Mature mouse Plexin C1 is 1540 amino acids (aa) in length. It contains a 916 aa extracellular domain that shows one Sema-domain (aa 35‑452) and a 603 aa cytoplasmic tail. There are two potential splice variants that show a five and 67 aa substitution for aa 861‑870 and 1206‑1574, respectively. Over aa 35‑950, mouse Plexin C1 is 95% and 85% aa identical to rat and human Plexin C1, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Plexin C1 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse Plexin C1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5375, filled histogram) or control antibody (Catalog # 5‑001‑A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). | |
Detection of Mouse Plexin C1 by Western Blot. Western blot shows lysates of mouse thymus tissue and embryonic mouse heart tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Plexin C1 at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Plexin C1 in Mouse Embryo. Plexin C1 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Plexin C1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5375) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neurites in the developing midbrain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
