Mouse IL-10 R beta Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-10 R beta Affinity Purified Polyclonal Ab (25 UG)

货号： AF5368-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF5368-SP
别名	CDw210b antigen; CDw210b; CRF2-4IL-10R subunit 2; CRFB4; CRFB4human transmembrane receptor protein; cytokine receptor10CDW210B; D21S58; D21S66; IBD25; IL-10 receptor subunit beta; IL-10R2; IL-10R2IL-10R subunit beta; IL10RB; IL-10RB; interleukin 10 receptor, beta; Interleukin-10 receptor subunit 2; interleukin-10 receptor subunit beta; member 4
全称	Interleukin 10 Receptor beta
反应种属	Mouse
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse IL-10 R beta in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human (rh) IL‑10 R beta is observed and less than 1% cross-reactivity with rhIL-10 R and recombinant mouse IL-10 R is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3588 (Human); 16155 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse IL-10 R beta Ile21-Ser220 Accession # Q61190
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-10 R beta (Interleukin 10 receptor beta; also IL‑10 R2, CD210b and CRF2-4) is a 60‑65 kDa member of the type II cytokine receptor family of proteins. It is very widely expressed, and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B and -29 (plus IL-26 in human). Mature mouse IL-10 R beta is a type I transmembrane protein that is 330 amino acids (aa) in length. It contains a 201 aa extracellular domain (aa 20‑220) that possesses two fibronectin type III domains (aa 22‑107 and 111‑208). There is one alternate start site at Met20. Over aa 21‑220, mouse IL-10 R beta shares 87% and 74% aa identity with rat and human IL-10 R beta, respectively.
运输条件	Blue Ice
存放说明	4℃
参考文献	B Cell Development and Differentiation Cytokines and Receptors in Allergy and Asthma Cytokines and Receptors in Angiogenesis Cytokines and Receptors in Tfh Cell Differentiation and Function IL-10/Interferon Family Monocyte Markers Neuroinflammation Receptors in the Jak/STAT Pathway Regulatory T Cells (Tregs)

参考图片

Detection of IL‑10 R beta in A20 Mouse Cell Line by Flow Cytometry. A20 mouse B cell lymphoma cell line was stained with Goat Anti-Mouse IL‑10 R beta Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5368, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108).

Detection of Mouse IL‑10 R beta by Western Blot. Western blot shows lysates of Differentiated 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL‑10 R beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5368) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL‑10 R beta at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.