| 货号 | AF5358-SP |
| 别名 | HL-2; intelectin 2; Intelectin-b; Itln1b; ITLN2; Itlnb; UNQ2789/PRO7179 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse Intelectin-2 in direct ELISAs and Western blots.In direct ELISAs, approximately 20% cross‑reactivity with recombinant mouse Intelectin-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 142683 (Human); 493583 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Intelectin-2 Ala20-Ser298 Accession # Q80ZA0 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Intelectin-2 (ITLN-2, ITLN-1b and HL-2) is a 37-41 kDa, likely Ca-dependent carbohydrate-binding lectin that is a member of the X-lectin family of molecules. It is secreted by airway mucous cells plus intestinal Paneth and goblet cells, and appears to participate in fungal microbe recognition. The mouse ITLN-2 precursor is 313 amino acids (aa) in length. It contains a 19 aa signal sequence, a 279 aa mature region (aa 20-298), and a GPI-linked prodomain (aa 299-313). The mature region contains one C-terminal type fibrinogen domain (aa 32-251). Over aa 20-298, mouse ITLN-2 shares 79% aa sequence identity with human ITLN-2, and 92% aa sequence identity with mouse ITLN-1. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Intelectin‑2 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Intelectin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5358) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Intelectin‑2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
