| 货号 | AF5330-SP |
| 别名 | Tnfrsf24; Tnfrsf26 | 全称 | Tumor Necrosis Factor Receptor Homolog 3 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse TNFRH3/TNFRSF26 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) TNF RI and rmTNF RII is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 244237 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant mouse TNFRH3/TNFRSF26 Ser20-Lys162 Accession # P83626 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TNFRH3 (Tumor necrosis factor receptor homolog 3; also TNFR superfamily member 26) is a 21 kDa (predicted) member of the TNF receptor superfamily. TNFRH3 is concentrated in lymphoid cell types. Mature mouse TNFRH3 is a type I transmembrane protein that is 185 amino acid (aa) in length. It contains a 145 aa extracellular region and a 19 aa cytoplasmic domain. The extracellular region contains three TNFR-Cys repeats that characterize TNF receptors (aa 27 - 143). Unlike two related TNF receptor homologs, TNFRH3 does not serve as a TRAIL decoy receptor. No ortholog to mouse TNFRH3 has been reported in human or rat. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of TNFRH3/TNFRSF26 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011). | |
Detection of Mouse TNFRH3/TNFRSF26 by Western Blot. Western blot shows lysates of mouse thymus tissue and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TNFRH3/TNFRSF26 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
