| 货号 | AF5206-SP |
| 别名 | IFNK | 全称 | Interferon kappa |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) |
| 目标/特异性 | Detects mouse IFN-kappa in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 56832 (Human); 387510 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse IFN-kappa Leu22-Lys199 (Cys174Tyr) Accession # Q7TSL0 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | IFN-kappa (Interferon kappa) is a secreted, nonglycosylated polypeptide that belongs to the IFN-kappa subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-kappa induces TNF-alpha, IL-10 and MCP-1 production by monocytes. Mouse IFN-kappa precursor is 199 amino acids (aa) in length. It contains a 21 aa signal sequence, plus a 178 aa mature region. There are two intrachain disulfide bonds plus a fifth unpaired cysteine. Mature mouse IFN-kappa (aa 22‑199) shares 68% and 30% aa identity with rat and human IFN-kappa, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse IFN-kappa by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, A20 mouse B cell lymphoma cell line, and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse IFN-kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IFN-kappa at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Mouse IFN‑ kappa by Simple WesternTM. Simple Western lane view shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and EL‑4 mouse lymphoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for IFN‑ kappa at approximately 40 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse IFN‑ kappa Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5206) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
