| 货号 | AF5160-SP |
| 别名 | Alkaline sphingomyelin phosphodiesterase; alkaline sphingomyelinase; Alk-SMase; EC 3.1.4.12; ectonucleotide pyrophosphatase/phosphodiesterase 7; ectonucleotide pyrophosphatase/phosphodiesterase family member 7; E-NPP 7; Intestinal alkaline sphingomyelinase; MGC50179; NPP7; NPP-7 | 全称 | Ectonucleotide Pyrophosphatase/Phosphodiesterase 7 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse ENPP‑7/Alk‑SMase in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human ENPP-7 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 339221 (Human); 238011 (Mouse); 303729 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ENPP‑7/Alk‑SMase Ala22-Gln421 Accession # NP_001025462 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ENPP7 (ectonucleotide pyrophosphatase/phosphodiesterase 7), also known as alkaline sphingomyelinase (Alk-SMase) is expressed in the intestines and in bile (1). It shares 30-36% homology with the members of the nucleotide pyrophosphatase/phosphodiesterese (NPP) family while sharing no similarities with neutral or acid SMase (2). Its main function is the digestion of dietary sphingomyelin by hydrolyzing sphingomyelin into ceramide and phosphorylcholine. ENPP7 is reported to hydrolyse and inactivate platelet-activating factor (PAF) by a phospholipase C-type activity (3). Studies show a decrease in ENPP7 activity in human colorectal adenocarcinomas and human colorectal carcinomas, which indicate a potential role of ENPP7 in human colon cancer (4, 5). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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ENPP‑7/Alk-SMase in intestine. ENPP‑7/Alk-SMase was detected in perfusion fixed frozen sections of mouse intestine using Mouse ENPP‑7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. | |
Detection of Mouse ENPP‑7/Alk-SMase by Western Blot. Western blot shows lysates of mouse small intestine tissue and rat small intestine tissue. PVDF membrane was probed with 1 µg/mL of Mouse ENPP‑7/Alk-SMase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5160) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ENPP‑7/Alk-SMase at approximately 60kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
