| 货号 | AF5046-SP |
| 别名 | B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein transcript; Bcl-5; BCL5B-cell lymphoma 5 protein; BCL-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; LAZ3ZBTB27B-cell lymphoma 6 protein; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27 ; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51ZNF51; zinc finger transcription factor BCL6S; ZNF51 | 全称 | B Cell Lymphoma/Leukemia 6 |
| 反应种属 | Human/Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human and mouse Bcl-6 in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 604 (Human); 12053 (Mouse); 303836 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Bcl-6 Glu522-Thr696 Accession # Q8CB25 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-6 (B-cell lymphoma protein 6; also ZFP51, LAZ-3 and Bcl-5) is an 80 kDa member of the Kruppel-like family of zinc finger proteins. It is expressed in germinal center B cells, neutrophils, monocytes, and immature dendritic cells, and serves as a potent transcriptional repressor of genes such as p53. Human Bcl-6 is 706 amino acids (aa) in length and contains an N-terminal BTB/POZ protein interaction domain (aa 32‑99) and a series of six C2H2-type C-terminal zinc finger domains that bind DNA (aa 518‑681). Centrally located are three PEST sequences (aa 336‑430) that undergo phosphorylation by MAPK. This increases the molecular weight of Bcl-6 to 97 kDa and induces its degradation. Human Bcl-6 is about 99% aa identical to mouse and canine Bcl-6. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Human Bcl‑6 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line, HeLa human cervical epithelial carcinoma cell line, and 293T human embryonic kidney cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Bcl‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Bcl‑6 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
