| 货号 | AF5015-SP |
| 别名 | canopy 4 homolog (zebrafish); CNPY4; MGC40499PRAT4BPRotein Associated with Tlr4; protein canopy homolog 4 | 全称 | Protein Associated with Toll-like Receptor 4B |
| 反应种属 | Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) |
| 目标/特异性 | Detects mouse and rat PRAT4B in Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PRAT4B and recombinant mouse PRAT4A is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 245812 (Human); 66455 (Mouse); 363886 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derive recombinant mouse PRAT4B Thr30-Leu245 Accession # Q8BQ47 |
| 生物活性 | Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PRAT4B (protein associated with TLR4-B; also Cnpy4, protein canopy homolog 4) is a member of the canopy family of proteins. It is expressed by diverse cell types, including macrophages, fibroblasts and B cells. PRAT4B appears to be both secreted, and retained internally, where it may stabilize immature TLR protein expression in the ER, and impact its appearance on the cell surface. Mouse PRAT4B is 245 amino acids (aa) in length. It contains a 27 aa signal sequence plus one coiled-coil region (aa 209 - 237). There is one potential splice variant that shows a 13 aa substitution for the C-terminal nine amino acids. Over aa 30 - 245, mouse PRAT4B is 95% and 87% aa identical to rat and human PRAT4B, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse and Rat PRAT4B by Western Blot. Western blot shows lysates of rat lung tissue and mouse spleen tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PRAT4B at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of PRAT4B in RAW264.7 Mouse Cell Line by Flow Cytometry RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse/Rat PRAT4B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5015, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). |
