| 货号 | AF496-SP |
| 别名 | EFNB2; ELF-2; Htk-L; LERK-5; NLERK-1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/106cells) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/106cells Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1948 (Human); 13642 (Mouse); 30219 (Zebrafish) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2 Arg27-Ala227 Accession # AAA82934 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse Ephrin‑B2 by Western Blot. Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ephrin‑B2 at approximately 40‑45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
Detection of Ephrin‑B2 in SH‑SY5Y Human Cell Line by Flow Cytometry. SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). | |
Ephrin‑B2 in Rat Hippocampal Neurons. Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
