| 货号 | AF4919-SP |
| 别名 | FLJ12795; FLJ13221; FLJ25756; IGDCC5; immunoglobulin superfamily, DCC subclass, member 5; Protein Shen-Dan; protogenin homolog (Gallus gallus); protogenin; PRTG; RPTG; Shen-Dan |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse and human PRTG in Western blots. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 283659 (Human); 235472 (Mouse); 315806 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse PRTG Val24-Thr943 Accession # Q2EY15 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Protogenin in Embryonic Mouse Neural Tube. Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. | |
Protogenin in Mouse Neural Tube. Protogenin was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
