| 货号 | AF4886-SP |
| 别名 | Acid ceramidase-like protein; ASAHL; ASAH-like protein; EC 3.5.1.-; NAAA; N-acylethanolamine acid amidase; N-acylethanolamine-hydrolyzing acid amidase; N-acylsphingosine amidohydrolase (acid ceramidase)-like; N-acylsphingosine amidohydrolase-like; PLT |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) ASAHL is observed and less than 5% cross-reactivity rhASAHL-2 and recombinant mouse ASAHL-2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 27163 (Human); 67111 (Mouse); 497009 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ASAHL/N‑acylethanolamine-hydrolyzing Acid A Val33-Ser362 Accession # AAH04572 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The mouse ASAHL gene encodes N-acylethanolamine-hydrolyzing Acid Amidase (NAAA), a fatty acid amidase with maximal activity at acidic pH (1). NAAA hydrolyzes a number of N-acyl ethanolamines, including N-myristoyl-,N-stearoyl-,N-oleoyl-, and N-arachidonoyl, but is most active against N-palmitoylethanolamine (2). NAAA is a member of the choloylglycine hydrolase family of enzymes, and is structurally similar to acid ceramidase (1). NAAA is both a lysosomal and a secreted enzyme, and like acid ceramidase, has been observed to be proteolytically processed during maturation (1). Through its amidase activity, ASAHL may play a role in the termination of the actions of a variety of N-acylethanolamides (3). NAAA can be distinguished from anandamide amidohydrolase by its lack of inhibition by methyl arachidonoyl fluorophosphonate (2). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4886) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for N-acylethanolamine-hydrolyzing Acid Amidase/ASAHL at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
