| 货号 | AF4804-SP |
| 别名 | Acig1; AIG1; Aig1L; BSRP-B; seizure related 6 homolog (mouse)-like; seizure related gene 6-like; SEZ6L | 全称 | Seizure-related 6 Homolog-like |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse SEZ6L in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-C is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 23544 (Human); 56747 (Mouse); 304554 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L Glu32-Ser889 Accession # AAH65117 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SEZ6L (Seizure 6-Like protein; also BSRP-B/Brain-specific receptor-like protein B) is a 200-210 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, particularly cerebellar interneurons plus Purkinje and granule cells. SEZ6L likely regulates Ca++ availability and thus impacts PKC alpha activation and mGluR1 signaling. Mature mouse SEZ6L is a 932 amino acid (aa) type I transmembrane protein. It contains an 866 aa extracellular domain (ECD) (aa 32-897) and a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There is one 190-200 kDa splice variant termed BSRP-B that contains a Phe substitution for aa 807-872 of the fifth SCR domain. Over aa 32-889, mouse SEZ6L shares 80% and 97% aa identity with human and rat SEZ6L, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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SEZ6L in Mouse Brain. SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
