Mouse CXCL13/BLC/BCA-1 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CXCL13/BLC/BCA-1 Affinity Purified Polyclonal Ab (25 UG)

货号： AF470-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF470-SP
别名	ANGIE; ANGIE2; B cell-attracting chemokine 1; B lymphocyte chemoattractant; BCA-1; BCA1B-cell chemoattractant; BCA-1CXC chemokine BLC; B-cell-homing chemokine (ligand for Burkitts lymphoma receptor-1); BLC; BLCSmall-inducible cytokine B13; BLR1L; B-lymphocyte chemoattractant; chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant); chemokine (C-X-C motif) ligand 13; C-X-C motif chemokine 13; SCYB13B-cell-attracting chemokine 1; small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize CXCL13/BLC/BCA‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR5. The Neutralization Dose (ND₅₀) is typically 20-50 µg/mL in the presence of 4 µg/mL Recombinant Mouse CXCL13/BLC/BCA‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	10563 (Human); 55985 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Stromal cells as trend-setters for cells migrating into the lymph node. Authors: Buettner M, Dittrich-Breiholz O, Falk C, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U Mucosal Immunol, 2015;8(3):640-9. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen IL-23 is required for long-term control of Mycobacterium tuberculosis and B cell follicle formation in the infected lung. Authors: Khader SA, Guglani L, Rangel-Moreno J J. Immunol., 2011;187(10):5402-7. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut. Authors: Suzuki K, Maruya M, Kawamoto S Immunity, 2010;33(1):71-83. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Lymphotoxin beta receptor signaling promotes tertiary lymphoid organogenesis in the aorta adventitia of aged ApoE-/- mice. Authors: Grabner R, Lotzer K, Dopping S, Hildner M, Radke D, Beer M, Spanbroek R, Lippert B, Reardon CA, Getz GS, Fu YX, Hehlgans T, Mebius RE, van der Wall M, Kruspe D, Englert C, Lovas A, Hu D, Randolph GJ, Weih F, Habenicht AJ J. Exp. Med., 2009;206(1):233-48. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Salmonella disrupts lymph node architecture by TLR4-mediated suppression of homeostatic chemokines. Authors: St John AL, Abraham SN Nat. Med., 2009;15(11):1259-65. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Role of lymphotoxin-alpha in cigarette smoke-induced inflammation and lymphoid neogenesis. Authors: Demoor T, Bracke KR, Maes T, Vandooren B, Elewaut D, Pilette C, Joos GF, Brusselle GG Eur. Respir. J., 2009;34(2):405-16. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Suppression of established experimental autoimmune encephalomyelitis and formation of meningeal lymphoid follicles by lymphotoxin beta receptor-Ig fusion protein. Authors: Columba-Cabezas S, Griguoli M, Rosicarelli B, Magliozzi R, Ria F, Serafini B, Aloisi F J. Neuroimmunol., 2006;179(1):76-86. Species: Mouse Sample Type: Whole Tissue Application: IHC Regulation of B1 cell migration by signals through Toll-like receptors. Authors: Ha SA, Tsuji M, Suzuki K, Meek B, Yasuda N, Kaisho T, Fagarasan S J. Exp. Med., 2006;203(11):2541-50. Species: Mouse Sample Type: Whole Tissue Application: IHC Fresh The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo. Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA J. Immunol., 2006;177(10):7296-302. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1 Ile22-Ala109 Accession # Q3U1E8
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.
运输条件	Blue Ice
存放说明	4℃
参考文献	Gunn, M.D. et al. (1998) Nature, 391:799. Legler, D.F. et al. (1998) J. Exp. Med. 187:655. Forster, R. et al. (1996) Cell 87:1037.

参考图片

Chemotaxis Induced by CXCL13/BLC/BCA‑1 and Neutralization by Mouse CXCL13/BLC/BCA‑1 Antibody. Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin staining. Chemotaxis elicited by Recombinant Mouse CXCL13/BLC/BCA‑1 (4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CXCL13/
BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470). The ND₅₀ is typically 20-50 µg/mL.

CXCL13/BLC/BCA‑1 in Mouse Intestine. CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyers patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.