| 货号 | AF4650-SP |
| 别名 | immunoglobulin superfamily containing leucine-rich repeat 2; KIAA1465; leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein; LINX; UNQ1885/PRO4329 | 全称 | Immunoglobulin Superfamily Containing Leucine-rich Repeat 2 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse ISLR-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human ISLR-2 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 57611 (Human); 320563 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ISLR-2 Cys20-Leu590 Accession # Q5RKR3 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ISLR2 (Ig‑superfamily containing LRR protein 2; also SST273) is a 100‑110 kDa member of the LRR family of proteins. It is expressed on developing motor, sensory and interneurons. Mouse ISLR2 is 745 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 571 aa extracellular domain (ECD) and a 132 aa cytoplasmic region. The ECD shows multiple LRRs (aa 21‑231) and one Ig‑like domain (aa 251‑374). Mouse ISLR2 apparently has one alternate start site at Met130. Over aa 20‑590, mouse ISLR2 is 89% and 98% aa identical to human and rat ISLR2, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of Mouse ISLR‑2 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and EL‑4 mouse lymphoblast cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse ISLR‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for ISLR‑2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
ISLR‑2 in Embryonic Mouse Brainstem. ISLR‑2 was detected in immersion fixed frozen sections of embryonic mouse brainstem (13 d.p.c.) using Sheep Anti-Mouse ISLR‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4650) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections. |
