Mouse TRANCE/RANK L/TNFSF11 Affinity Purifed Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse TRANCE/RANK L/TNFSF11 Affinity Purifed Polyclonal Ab (25 UG)

货号： AF462-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF462-SP
别名	CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
全称	TNF-related Activation-induced Cytokine
反应种属	Mouse
应用	Western Blot,Immunohistochemistry,ELISA Capture (Matched Antibody Pair),ELISA Detection (Matched Antibody Pair),ELISA Standard ,Neutralization
目标/特异性	Detects mouse TRANCE/TNFSF11/RANK L in ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) CD27, rmCD40, and rmFas is observed and less than 1% cross-reactivity with rmCD30, recombinant human (rh) DR6, rmGITR, rmTNF RI, and rmTNF RII is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL ELISA Capture (Matched Antibody Pair): 0.2-0.8 µg/mL ELISA Detection (Matched Antibody Pair): 0.1-0.4 µg/mL ELISA Standard : Neutralization: Measured by its ability to neutralize TRANCE/TNFSF11/RANK L-induced osteoclast-like cell formation in mouse splenocytes. Nakagawa, N. et al. (1998) Biochim. Biophys. Res. Commun. 253:395. The Neutralization Dose (ND₅₀) is typically 0.02-0.06 µg/mL in the presence of 30 ng/mL Recombinant Mouse TRANCE/TNFSF11/RANK L.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	8600 (Human); 21943 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Fibromodulin and Biglycan Modulate Periodontium through TGFbeta/BMP Signaling. Authors: Wang L, Foster B, Kram V, Nociti F, Zerfas P, Tran A, Young M, Somerman M J Dent Res, 2014;93(8):780-787. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded CXCL5 stimulation of RANK ligand expression in Paget's disease of bone. Authors: Sundaram K, Rao D, Ries W, Reddy S Lab Invest, 2013;93(4):472-9. Species: Human Sample Type: Cell Lysates Application: WB Osteoclast differentiation factor RANKL controls development of progestin-driven mammary cancer. Authors: Schramek D, Leibbrandt A, Sigl V, Kenner L, Pospisilik JA, Lee HJ, Hanada R, Joshi PA, Aliprantis A, Glimcher L, Pasparakis M, Khokha R, Ormandy CJ, Widschwendter M, Schett G, Penninger JM Nature, 2010;468(7320):98-102. Species: Mouse Sample Type: Cell Lysates Application: WB The RANKL signaling axis is sufficient to elicit ductal side-branching and alveologenesis in the mammary gland of the virgin mouse. Authors: Fernandez-Valdivia R, Mukherjee A, Ying Y, Li J, Paquet M, DeMayo FJ, Lydon JP Dev. Biol., 2009;328(1):127-39. Species: Mouse Sample Type: Cell Lysates Application: WB Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice. Authors: Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL Circulation, 2008;117(3):411-20. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded Estrogen and progesterone are critical regulators of Stat5a expression in the mouse mammary gland. Authors: Santos SJ, Haslam SZ, Conrad SE Endocrinology, 2007;149(1):329-38. Species: Mouse Sample Type: Whole Tissue Application: IHC Paraffin-embedded IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance. Authors: Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB J. Immunol., 2003;170(5):2655-62. Species: Mouse Sample Type: Synovial Fluid Application: ELISA Development Paracrine-rescued lobulogenesis in chimeric outgrowths comprising progesterone-receptor-null mammary epithelium and redirected wild-type testicular cells. Authors: Bruno R, Boulanger C, Rosenfield S, Anderson L, Lydon J, Smith G J Cell Sci, 0;127(0):27-32. Species: Mouse Sample Type: Whole Tissue Application: IHC - Paraffin embedded
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant mouse TRANCE/TNFSF11/RANK L Arg72-Asp316 Accession # O35235
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokines, osteoprotegerin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. In the TNF superfamily nomenclature, TRANCE is referred to as TNFSF11. TRANCE was originally identified as an immediate early gene upregulated by T cell receptor stimulation. The murine TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegerin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.
运输条件	Blue Ice
存放说明	4℃
参考文献	Wong, B.R.et al. (1997) J. Biol. Chem. 272:25190. Anderson, D.M. et al. (1997) Nature 390:175. Nakagawa, N.et al. (1998) Biochem. Biophys. Res. Commun. 245:382. Kong, Y-Y. et al. (1999) Nature 397:315.

参考图片

Osteoclast-like Cell Formation Induced by TRANCE/
TNFSF11/RANK L and Neutralization by Mouse TRANCE/TNFSF11/RANK L Antibody. In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TR) induces osteoclast-like cell formation in mouse splenocytes in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) solution assay. Under these conditions, osteoclast-like cell formation elicited by Recombinant Mouse TRANCE/TNFSF11/
RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TRANCE/TNFSF11/RANK L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF462). The ND₅₀ is typically 0.02-0.06 µg/mL.