| 货号 | AF454-SP |
| 别名 | Eek; EK3; EPH- and ELK-related kinase; EPH- and ELK-related tyrosine kinase; EPH receptor A8; EPH-like kinase 3; ephrin type-A receptor 8; Hek3; hydroxyaryl-protein kinase; KIAA1459; protein-tyrosine kinase; tyrosine-protein kinase receptor EEK; tyrosylprotein kinase | 全称 | Eph Receptor A8 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects recombinant mouse EphA8 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant rat EphA5, recombinant mouse (rm) EphA6, rmEphA7, rmEphA2, recombinant human EphA1, and rmEphA3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2046 (Human); 13842 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse EphA8 Gly27 - Arg540 Accession # O09127 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EphA8, also known as Eek and Hek3 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA8 has been shown to bind ephrin‑A5, ephrin‑A3, and ephrin‑A2 (2, 3). The full-length human and rat homologs have not been cloned. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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