| 货号 | AF4518-SP |
| 别名 | c-erbB3; EC 2.7.10; EC 2.7.10.1; ErbB-3; erbB3-S; HER3; HER3c-erbB-3; LCCS2; lethal congenital contracture syndrome 2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; Proto-oncogene-like protein c-ErbB-3; receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian) | 全称 | Receptor Tyrosine Protein Kinase ErbB3 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse ErbB3/Her3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human ErbB3 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2065 (Human); 13867 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse ErbB3/Her3 Ser20-His641 Accession # Q61526 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ErbB3, also called Her3 (human epidermal growth factor receptor 3) in humans, is a type I transmembrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors named for a viral oncogene (1‑3). ErbB family members serve as receptors for the EGF family of growth factors (1‑3). Mouse ErbB3 contains a 19 amino acid (aa) signal sequence, a 622 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 677 aa cytoplasmic domain (4). Human ErbB3 has four isoforms created by intron read-through and truncation of the molecule (5). Three of these are secreted and at least one can inhibit ErbB3 activity (6). Little information is available concerning mouse ErbB3 isoforms. The mouse ErbB3 ECD shares 97%, 93%, 92%, 91%, 89% and 88% aa identity with rat, human, bovine, equine, canine and opossum ErbB3, respectively. ErbB3 is found in epithelial cell layers of gastrointestinal, reproductive, urinary, endocrine and nervous systems, skin and muscle (3). Among ErbB family members, only ErbB3 lacks a working kinase domain, requiring heterodimerization with another ErbB receptor for signaling (1‑3). The heterodimer of ErbB3 with ErbB2, which has no known ligands of its own, is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2 (3, 7‑9). These ligands also bind ErbB4 (1). Signaling is aided by the six consensus binding motifs for the SH2 domain and one for the SH3 domain of the regulatory p85 subunit of phosphoinositide 3-kinase (10, 11). Deletion studies in mice demonstrate non-redundant roles for ErbB3 in development of Schwann cells, neural crest cells and heart valves (12, 13). ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system (14). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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Detection of ErbB3/Her3 in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Sheep Anti-Mouse ErbB3/Her3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4518, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). | |
ErbB3/Her3 in Embryonic Mouse Dorsal Root Ganglion. ErbB3/Her3 was detected in immersion fixed frozen sections of embryonic mouse dorsal root ganglion (E10.5) using 10 µg/mL Mouse ErbB3/Her3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4518) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. |
