Mouse IL-17 RA/IL-17 R Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-17 RA/IL-17 R Affinity Purified Polyclonal Ab (25 UG)

货号： AF448-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF448-SP
别名	CD217 antigen; CD217; Cdw217; CDw217interleukin 17 receptor; hIL-17R; IL-17 R; IL-17 receptor A; IL17RA; IL-17RAMGC10262; IL17Rinterleukin-17 receptor A; interleukin 17 receptor A
全称	Interleukin 17 Receptor
反应种属	Mouse
应用	Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects mouse IL‑17 RA/IL‑17 R in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑17 RA/IL‑17 R is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-17 RC and rmIL-17 RD is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	23765 (Human); 16172 (Mouse); 312679 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. TLR2 deficiency leads to increased Th17 infiltrates in experimental brain abscesses. Authors: Nichols JR, Aldrich AL, Mariani MM, Vidlak D, Esen N, Kielian T J. Immunol., 2009;182(11):7119-30. Species: Mouse Sample Type: Whole Cells Application: Flow IL-17RD (Sef or IL-17RLM) interacts with IL-17 receptor and mediates IL-17 signaling. Authors: Rong Z, Wang A, Li Z, Ren Y, Cheng L, Li Y, Wang Y, Ren F, Zhang X, Hu J, Chang Z Cell Res., 2009;19(2):208-15. Species: Mouse Sample Type: Cell Lysates Application: IP IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity. Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW J. Immunol., 2009;182(12):7398-407. Species: Mouse Sample Type: Whole Cells Application: Flow IL-17 receptor signaling influences virus-induced corneal inflammation. Authors: Molesworth-Kenyon SJ, Yin R, Oakes JE, Lausch RN J. Leukoc. Biol., 2007;83(2):401-8. Species: Mouse Sample Type: Whole Cells Application: ICC Inhibition of interleukin-17 prevents the development of arthritis in vaccinated mice challenged with Borrelia burgdorferi. Authors: Burchill MA, Nardelli DT, England DM, DeCoster DJ, Christopherson JA, Callister SM, Schell RF Infect. Immun., 2003;71(6):3437-42. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Antigen Affinity-purified
免疫原	S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse IL‑17 RA/IL‑17 R Extracellular domain
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	IL-17 R, also known as IL-17 RA, is a 120 kDa type I transmembrane glycoprotein protein that plays a central role in inflammatory responses (1-3). Mature mouse IL‑17 R consists of a 291 amino acid (aa) extracellular domain, a 21 aa transmembrane segment, and a 521 aa cytoplasmic domain (4). The cytoplasmic domain contains a region homologous to the TIR domain of the TLR/IL-1 R family (5). Mouse IL-17 R shares 84% and 72% aa sequence identity with rat and human IL-17 R, respectively. Within the extracellular domain, it shares 18-25% sequence identity with mouse IL-17 RB, C, D, and E. While the expression of IL-17 is restricted to activated T cells, IL-17 R exhibits a broad tissue distribution (4). Even in the absence of ligand, IL-17 R exists on the cell surface as a multimer (6). IL-17 R can bind IL-17 but must associate with IL-17 RC to transduce signals (7, 8). Interestingly, human IL-17 R does not appear to form productive complexes with mouse IL-17 RC (8). The IL-17 R can also signal in response to IL-17F (9). IL-17 R ligation promotes T cell activation and the production of IL-6, G-CSF, SCF, and multiple pro‑inflammatory chemokines (4, 7, 9, 10). IL-17A and IL-17F synergize with TNF-alpha in the induction of CXCL1, G-CSF, and IL-6 (9, 11). This effect requires the presence of both TNF RI and TNF RII (9). IL-17 interactions with IL-17 R also inhibit the TNF-alpha induced upregulation of fibroblast CCL5 and VCAM-1 (11). CCL5 and VCAM-1 induced effects are differentially sensitive to blockade with IL-17 R specific antibodies, suggesting that IL-17 R triggers divergent intracellular signals (11). In vivo, IL‑17 R activity is important for increased generation of neutrophils and their recruitment to sites of inflammation (10, 12, 13). IL-17 R is required for host defense against microbial infection and for the progression of arthritis from inflammation to destructive joint erosion (10, 13).
运输条件	Blue Ice
存放说明	4℃
参考文献	Iwakura, Y. and H. Ishigame (2006) J. Clin. Invest. 116:1218. Moseley, T.A. et al. (2003) Cytokine Growth Factor Rev. 14:155. Kawaguchi, M. et al. (2004) J. Allergy Clin. Immunol. 114:1265. Yao, Z. et al. (1995) Immunity 3:811. Novatchkova, M. et al. (2003) Trends Biochem. Sci. 28:226. Kramer, J.M. et al. (2006) J. Immunol. 176:711. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332. Toy, D. et al. (2006) J. Immunol. 177:36. McAllister, F. et al. (2005) J. Immunol. 175:404. Ye, P. et al. (2001) J. Exp. Med. 194:519. Schnyder, B. et al. (2005) Cytokine 31:191. Tan, W. et al. (2006) J. Immunol. 176:6186. Lubberts, E. et al. (2005) J. Immunol. 175:3360.

参考图片

Detection of Mouse IL‑17 RA/IL‑17 R by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse IL‑17 RA/IL‑17 R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF448) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑17 RA/IL‑17 R at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of IL‑17 RA/IL‑17 R in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Goat Anti-Mouse IL‑17 RA/IL‑17 R Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF448, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).