Mouse IL-15 Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse IL-15 Affinity Purified Polyclonal Ab (25 UG)

货号： AF447-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF447-SP
别名	IL-15MGC9721; interleukin 15; interleukin-15
全称	Interleukin 15
反应种属	Mouse
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects mouse IL-15 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross-reactivity with recombinant human IL‑15 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑15-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359. The Neutralization Dose (ND₅₀) is typically 2.5-10 µg/mL in the presence of 0.3 µg/mL Recombinant Mouse IL‑15.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3600 (Human); 16168 (Mouse); 25670 (Rat); 493682 (Feline)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Transcription factor KLF2 regulates homeostatic NK cell proliferation and survival Authors: W Rabacal, SK Pabbisetty, KL Hoek, D Cendron, Y Guo, D Maseda, E Sebzda Proc Natl Acad Sci USA, 2016;0(0):. Species: Mouse Sample Type: Whole Tissue Application: IHC - Frozen ESAT-6-dependent cytosolic pattern recognition drives noncognate tuberculosis control in vivo Authors: A Kupz, U Zedler, M Stäber, C Perdomo, A Dorhoi, R Brosch, SH Kaufmann J Clin Invest, 2016;0(0):. Species: Mouse Sample Type: In Vivo Application: Neut Combination of TACI-IgG and anti-IL-15 treats murine lupus by reducing mature and memory B cells. Authors: Ma N, Xiao H, Marrero B, Xing C, Wang X, Zheng M, Han G, Chen G, Hou C, Shen B, Li Y, Wang R, Jiang Z Cell Immunol, 2014;289(1):140-4. Species: Mouse Sample Type: In Vivo Application: Neut Blockade of IL-15 activity inhibits microglial activation through the NFkappaB, p38, and ERK1/2 pathways, reducing cytokine and chemokine release. Authors: Gomez-Nicola D, Valle-Argos B, Nieto-Sampedro M Glia, 2010;58(3):264-76. Species: Mouse Sample Type: Whole Cells Application: ICC IL-15Ralpha chaperones IL-15 to stable dendritic cell membrane complexes that activate NK cells via trans presentation. Authors: Mortier E, Woo T, Advincula R, Gozalo S, Ma A J. Exp. Med., 2008;205(5):1213-25. Species: Mouse Sample Type: Cell Lysates Application: ELISA Development A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex. Authors: Rafei M, Wu JH, Annabi B, Lejeune L, Francois M, Galipeau J Blood, 2007;109(5):2234-42. Species: Mouse Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse IL-15 Asn49-Ser162 Accession # P48346
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 (1-3). Mature mouse IL-15 shares 70% and 96% amino acid sequence identity with human and rat IL-15, respectively. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (4). IL-15 binds with high affinity to IL-15 R alpha (5). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (1, 6). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (7, 8). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (9). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (8, 10). Soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding or alternate splicing (11-13). These molecules retain the ability to bind tightly to IL-15 and can either inhibit or augment IL-15 function (5, 12, 13). Consistent with its shared use of IL-2 receptor subunits, IL-15 induces IL-2-like effects in lymphocyte development and homeostasis (3). It is particularly important for the maintenance and activation of NK cells and CD8⁺ memory T cells (3). IL-15 also exerts pleiotropic effects on other hematopoietic cells and non-immune cells (2). Ligation of membrane-associated IL-15/IL-15 R alpha complexes induces reverse signaling that promotes cellular adhesion, tyrosine phosphorylation of intracellular proteins, and cytokine secretion by the IL-15/IL-15 R alpha expressing cells (14, 15).
运输条件	Blue Ice
存放说明	4℃
参考文献	Grabstein, K. et al. (1994) Science 264:965. Budagian, V. et al. (2006) Cytokine Growth Factor Rev. 17:259. Ma, A. et al. (2006) Annu. Rev. Immunol. 24:657. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444. Giri, J.G. et al. (1995) EMBO 14:3654. Giri, J. et al. (1994) EMBO J. 13:2822. Duitman, E.H. et al. (2008) Mol. Cell. Biol. 28:4851. Dubois, S. et al. (2002) Immunity 17:537. Stonier, S.W. and K.S. Schluns (2010) Immunol. Lett. 127:85. Burkett, P.R. et al. (2004) J. Exp. Med. 200:825. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368. Mortier, E. et al. (2004) J. Immunol. 173:1681. Bulanova, E. et al. (2007) J. Biol. Chem. 282:13167. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192. Neely, G.G. et al. (2004) J. Immunol. 172:4225.

参考图片

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse IL‑15 Antibody. Recombinant Mouse IL‑15 (Catalog # 447-ML) stimulates proliferation in the CTLL‑2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑15 (0.3 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447). The ND₅₀ is typically 2.5-10 µg/mL.

IL‑15 in Mouse Intestine. IL‑15 was detected in immersion fixed frozen sections of mouse intestine (Peyer patch) using Goat Anti-Mouse IL‑15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF447) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes in villi. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.