| 货号 | AF4429-SP |
| 别名 | CAG4A; canopy 3 homolog (zebrafish); CNPY3; CTG4A; ERDA5; ERDA5CTG repeat protein 4a; Expanded repeat-domain protein CAG/CTG 5; PRAT4ACAG repeat containing; Protein associated with TLR4; protein associated with Toll-like receptor 4A; protein canopy homolog 3; TNRC5; TNRC5expanded repeat domain, CAG/CTG 5; trinucleotide repeat containing 5; Trinucleotide repeat-containing gene 5 protein | 全称 | Protein Associated with Toll-like Receptor 4A |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects mouse PRAT4A in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant mouse PRAT4B is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10695 (Human); 72029 (Mouse); 316225 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endoplasmic Protein Nogo-B (RTN4-B) Interacts with GRAMD4 and Regulates TLR9-Mediated Innate Immune Responses. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse PRAT4A isoform 1 Glu38-Leu276 Accession # Q9DAU1 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | PRAT4A (Proteinassociated with TLR4-A) is a ubiquitously expressed, 40 kDa protein that participates in Toll receptor expression. Mouse PRAT4A is 276 amino acids (aa) in length. It contains a putative signal sequence of 37 aa, followed by a 239 aa mature region. Although the presence of a signal sequence suggests it is secreted, its best known activity involves an intracellular association with TLR4. PRAT4A appears to facilitate the maturation and expression of TLR4 on the cell surface. Studies suggest this involves the C-terminal 60 amino acids. Mature mouse PRAT4A shares 90% and 96% aa identity with human and rat PRAT4A, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
|
Detection of Mouse PRAT4A by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Mouse PRAT4A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4429) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PRAT4A at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
