Mouse Haptoglobin Affinity Purified Polyclonal Ab (25 UG)
货号：
AF4409-SP 基本售价：
1378.1 元 规格：
-
产品信息
概述| 货号 | AF4409-SP |
| 别名 | binding peptide; BP; haptoglobin alpha(1S)-beta; haptoglobin alpha(2FS)-beta; haptoglobin; haptoglobin, alpha polypeptide; haptoglobin, beta polypeptide; HP; HP-1; hp2-alpha; HP2ALPHA2; HPA1S; MGC111141 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1 µg/mL)
|
| 目标/特异性 | Detects mouse Haptoglobin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with human Haptoglobin is observed. |
| 使用方法 | Western Blot: 1 µg/mL
|
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 3240 (Human); 15439 (Mouse); 24464 (Rat) |
| 应用文献 |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Dimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease
Authors: S Krishnamoo, B Pace, D Gupta, S Sturtevant, B Li, L Makala, J Brittain, N Moore, BF Vieira, T Thullen, I Stone, H Li, WE Hobbs, DR Light
JCI Insight, 2017;2(20):.
Species: Mouse
Sample Type: Tissue Lysates
Application: WB |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse serum-derived Haptoglobin |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Haptoglobin is a 45‑48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin. Mouse (pro)haptoglobin shares 90% and 80% aa identity with rat and human (pro)haptoglobin, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 | - Acute Phase Proteins
- Anti-oxidant Molecules
- Diabetes
- Obesity
- Oxidative Stress
- Serine Proteases and Regulators
|
参考图片Detection of Mouse Haptoglobin by Western Blot. Western blot shows samples of purified mouse Haptoglobin (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4409) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Haptoglobin was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
