Mouse CCL22/MDC Affinity Purified Polyclonal Ab (25 UG)【科瑞奥博】

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Mouse CCL22/MDC Affinity Purified Polyclonal Ab (25 UG)

货号： AF439-SP 基本售价： 1378.1 元规格： -

产品信息

概述
货号	AF439-SP
别名	A-152E5.1; ABCD-1; CC chemokine STCP-1; C-C motif chemokine 22; chemokine (C-C motif) ligand 22; DC/B-CK; Macrophage-derived chemokine; MDC; MDCStimulated T-cell chemotactic protein 1; MGC34554; SCYA22MDC(1-69); small inducible cytokine A22; small inducible cytokine subfamily A (Cys-Cys), member 22; Small-inducible cytokine A22; STCP-1; stimulated T cell chemotactic protein 1
反应种属	Mouse
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND₅₀) is typically 0.3-1.0 µg/mL in the presence of 15 ng/mL Recombinant Mouse CCL22/MDC.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6367 (Human); 20299 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets. Authors: Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB J. Clin. Invest., 2011;121(0):3024. Species: Mouse Sample Type: Cell Lysates Application: WB Comprehensive assessment of chemokine expression profiles by flow cytometry. Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D J. Clin. Invest., 2010;120(3):907-23. Species: Mouse Sample Type: Whole Cells Application: Flow Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells. Authors: Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ J. Exp. Med., 2008;205(3):699-710. Species: Mouse Sample Type: In Vivo Application: In vivo The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells. Authors: Fujita H, Asahina A, Komine M, Tamaki K Cell. Immunol., 2007;245(2):70-9. Species: Mouse Sample Type: Cell Culture Supernates Application: Functional Assay Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis. Authors: Carpenter KJ, Hogaboam CM Infect. Immun., 2005;73(11):7198-207. Species: Mouse Sample Type: Application: Neut
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant mouse CCL22/MDC Gly25-Ser92 Accession # Q9QZU2
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Mouse ABCD-1, the mouse orthologue of the human macrophage-derived chemokine (MDC)/stimulated T cell chemotactic protein (STCP-1), is a CC chemokine cloned from activated mouse B cells. Mouse MDC cDNA encodes a precursor protein of 92 amino acid (aa) residues with a 24 aa residue predicted signal peptide that is cleaved to yield a 68 aa residue mature 7.8 kDa protein. At the amino acid sequence level, mouse and human MDC share 64% identity and 83% similarity. The genomic organization of the mouse and human MDC genes are very similar, exhibiting sequence identity at the intron-exon boundaries. Mouse MDC is expressed at high levels in dendritic cells and activated B lymphocytes. Low levels of mouse MDC mRNA are also detectable in lung, unstimulated spleen cells, lymph node cells and in thymocytes. MDC is a functional ligand for the CC chemokine receptor 4. Recombinant or chemically synthesized mature mouse MDC has been shown to induce chemotaxis or Ca²⁺ mobilization in activated mouse and human T cells.
运输条件	Blue Ice
存放说明	4℃
参考文献	Schaniel, C. et al. (1998) J. Exp. Med. 188:451. Imai, T. et al. (1998) J. Biol. Chem. 273:1764. Godiska, R. et al. (1997) J. Exp. Med. 185:1595. Chang, M-S. et al. (1997) J. Biol. Chem. 272:25229.

参考图片

Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439‑MD) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND₅₀ is typically 0.3-1.0 µg/mL.