| 货号 | AF4298-SP |
| 别名 | EGFL6L; Nctn; nephronectin; NPNT; POEM |
| 反应种属 | Mouse |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Nephronectin in direct ELISAs. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 255743 (Human); 114249 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Nephronectin Asp20-Cys561 Accession # AAH68308 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Nephronectin, also named POEM (preosteoblast EGF repeat protein with MAM domain) is a 70‑90 kDa extracellular matrix protein that is a ligand for integrin alpha 8beta1(1, 2). The 561 amino acid (aa) mouse Nephronectin (isoform b) contains a 19 aa signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like Nephronectin, mediate cell adhesion and spreading (1, 2). Isoform a includes a 17 aa insertion between aa 58 and 59 that is missing in isoform b. Mouse Nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, bovine and opossum Nephronectin, respectively. It is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle (1, 2). Pre-osteoblast cells produce Nephronectin, but down‑regulate it as they differentiate (1). It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in-vivo complex with integrin alpha 8beta1(2). Since deletion of integrin alpha 8beta1 results in renal agenesis, Nephronectin has been proposed to be a critical integrin alpha 8beta1 ligand during Nephrogenesis (2, 3). |
| 运输条件 | Blue Ice |
| 存放说明 | 4℃ |
| 参考文献 |
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